Assessing kinetic and epitopic diversity across orthogonal monoclonal antibody generation platforms
نویسندگان
چکیده
منابع مشابه
Assessing kinetic and epitopic diversity across orthogonal monoclonal antibody generation platforms
The ability of monoclonal antibodies (mAbs) to target specific antigens with high precision has led to an increasing demand to generate them for therapeutic use in many disease areas. Historically, the discovery of therapeutic mAbs has relied upon the immunization of mammals and various in vitro display technologies. While the routine immunization of rodents yields clones that are stable in ser...
متن کاملNext-generation antibody discovery platforms.
A ntibody discovery platforms have emerged in recent years as an important source of both therapeutic molecules and research reagents. In PNAS, Larman et al. (1) provide the foundation for the next generation of antibody production using a rationally designed, fully defined scFv library and analysis pipeline, which is optimized for analysis with short-read deep-sequencing technologies. DNA sequ...
متن کاملGeneration and Evaluation of Monoclonal Autoantibodies in Systemic Lupus Erythematosis
Three murine monoclonal anti-ssDNA autoantibodies have been produced by fusion of spleen cells from NZB/NZW F1 hybrid mice with Sp2/0-Ag 14 murine myeloma cells. Specificity studies characterizing monoclonal anti - ssDNA autoantibodies revealed binding patterns for nucleotides. Clone E-4-1 showed preferential binding with mono-di-and polyguanosine. Clones E-4-4 and E-11-1 specitically bound mon...
متن کاملGeneration and evaluation of an anti-REV1 monoclonal antibody.
Continuous exposure of cells to exogenous and endogenous agents produces many types of DNA damage during normal cell cycles. Post-replication repair, consisting of error-free and error-prone sub-pathways, is required for tolerance of such DNA damage. REV1 plays a crucial role in regulation of the error-prone pathway. To facilitate analysis of its cellular functions, we here generated a mouse Re...
متن کاملGeneration of monoclonal antibody against canine neural-cell adhesion molecule.
A monoclonal antibody, K9BYU, was generated using Escherichia coli recombinant extracellular domain of canine neural-cell adhesion molecule (N-CAM) as an antigen. Immunoreactivity of K9BYU to insect cell recombinant canine N-CAM was demonstrated by Western blotting using Sf9 insect cells transfected with the canine N-CAM gene. In Western blotting against canine brain tissue, K9BYU detected thre...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: mAbs
سال: 2015
ISSN: 1942-0862,1942-0870
DOI: 10.1080/19420862.2015.1118596